Thermo Fisher SureTect Escherichia coli STEC Identification PCR Assay, 96T, A56840

The Thermo Scientific™ SureTect™ Escherichia coli STEC Identification PCR Assay is part of a complete workflow for the rapid detection of Escherichia coli O157:H7, stx, eae, and the Escherichia coli STEC serotypes (O26, O45, O103, O111, O121, O145) from food samples. The assay incorporates an optimized liquid lysis step, providing a quick and simple method for preparing the sample for rapid detection on the Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System and software.

Samples assessed as positive using the SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay are further tested using this SureTect™ Escherichia coli STEC Identification PCR Assay. The assay has been AOAC-RI and AFNOR (ISO 16140-2:2016) validated on a range of food, including low and high sample sizes of raw meat (excluding poultry), vegetables, fruits, juices, and dairy samples, and it enables users to reliably detect and differentiate Shiga-toxin producing Escherichia coli O157:H7 and other STEC in as little as 10 hours.